In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide

F Separovic; V Hofferek; AP Duff; MJ McConville; MA Sani

Journal article

In-cell DNP NMR reveals multiple targeting effect of antimicrobial peptide

F Separovic, V Hofferek, AP Duff, MJ McConville, MA Sani

Journal of Structural Biology X | ELSEVIER | Published : 2022

DOI: 10.1016/j.yjsbx.2022.100074

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Abstract

Dynamic nuclear polarization NMR spectroscopy was used to investigate the effect of the antimicrobial peptide (AMP) maculatin 1.1 on E. coli cells. The enhanced 15N NMR signals from nucleic acids, proteins and lipids identified a number of unanticipated physiological responses to peptide stress, revealing that membrane-active AMPs can have a multi-target impact on E. coli cells. DNP-enhanced 15N-observed 31P-dephased REDOR NMR allowed monitoring how Mac1 induced DNA condensation and prevented intermolecular salt bridges between the main E. coli lipid phosphatidylethanolamine (PE) molecules. The latter was supported by similar results obtained using E. coli PE lipid systems. Overall, the abil..

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University of Melbourne Researchers

Frances Separovic Author

Vinzenz Hofferek Author

Malcolm McConville Author

Marco Sani Author

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Grants

Awarded by Australian Research Council

Funding Acknowledgements

Acknowledgements This work was supported by the Australian Research Council [LE160100120, DP160100959, DP190101506 and DP210101792] . Ac- cess to NMR spectrometers was provided by the Bio21 Institute at the University of Melbourne. The National Deuteration Facility is partly supported by the National Collaborative Research Infrastructure Strat- egy - an initiative of the Australian Government.